Gene Targeting & Embryonic Stem Cells Paperback

Gene Targeting and Embryonic Stem Cells serves as a comprehensive manual for the increasing number of scientists entering this dynamic area of research. It offers step-by-step instructions on culturing, transfecting, and differentiating established cell lines, as well as methods for isolating novel lines. The guide presents gene targeting procedures for various cell types, including murine embryonic stem (ES) cells, human ES and embryonal carcinoma cells, and fetal fibroblasts from ungulates. Protocols include the construction of gene-targeting vectors, techniques for DNA transfection, and the use of RNA interference. With the recent derivation of human embryonic stem cells and their promising therapeutic potential, a new set of research tools and techniques has emerged. Likewise, gene targeting has evolved to include nuclear donor cells in ungulate species. This resource is particularly useful for both novice and seasoned investigators working with human ES cells, as well as for biotech firms focused on producing transgenic livestock, advancing xenotransplantation, and developing animal disease models.