Optimization In Drug Discovery In Vitro Methods 2Ed (Hb 2014) Hardcover

Thoroughly revised and updated, Optimization in Drug Discovery: In Vitro Methods, Second Edition provides a comprehensive overview of a wide range of in vitro assays essential for drug discovery. These include formulation studies, plasma protein binding, absorption and permeability testing, cytochrome P450 (CYP) and UDP-glucuronosyltransferases (UGT) metabolism, CYP inhibition and induction, drug transporters, drug-drug interactions through reactive metabolite assessment, as well as genotoxicity, chemical mutagenicity, and photo-mutagenicity assays. As part of the Methods in Pharmacology and Toxicology series, each chapter introduces its respective topic, outlines necessary materials and reagents, presents step-by-step experimental protocols, and offers troubleshooting tips to help avoid common pitfalls. Developed by expert authors with extensive experience in optimizing in vitro assays, this book focuses on achieving “drug-like” properties while ensuring efficacy and safety in drug candidates. With its comprehensive and up-to-date content, this second edition serves as a valuable resource for researchers navigating the complex process of drug discovery and development.